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Unique Properties of Nucleic Acid from Bacillus subtilis Phage SP-15

Nature New Biology volume 239, pages 68–70 (1972)Cite this article

Abstract

BACTERIOPHAGE SP-15 is a large, generalized transducing phage of Bacillus subtilis and B. licheniformis. The DNA extracted from the purified phage has unusual physical properties: its melting temperature (Tm) in 0.15 M NaCl, 0.015 M sodium citrate (SSC) is very low, 61.5° C, and its buoyant density in neutral CsCl is very high, 1.761 g/ml.1. We describe here additional unique features of SP-15 DNA: the presence of (1) a new modified pyrimidine which partially replaces the thymine; (2) a compound which reacts with orcinol as a pentose; (3) alkali-sensitive phosphodiester bonds; and (4) glucose.

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Authors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461

    J. MARMURM, C. BRANDON, S. NEUBORT & M. EHRLICH

  2. Department of Biology, University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas, 77025

    M. MANDEL & J. KONVICKA

Authors
  1. J. MARMURM
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  2. C. BRANDON
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  3. S. NEUBORT
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  4. M. EHRLICH
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  5. M. MANDEL
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  6. J. KONVICKA
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MARMURM, J., BRANDON, C., NEUBORT, S. et al. Unique Properties of Nucleic Acid from Bacillus subtilis Phage SP-15. Nature New Biology 239, 68–70 (1972). https://doi.org/10.1038/newbio239068a0

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  • DOI: https://doi.org/10.1038/newbio239068a0

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