The antibacterial properties of anise oil are useful against strains such as Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Corynebacterium ovis. Anethole, the predominant active ingredient of anise, has antimicrobial and antifungal properties, which is useful against Candida albicans and fungal strains such as Alternaria, Aspergillus, Cladosporium, Fusarium and Penicillin.